Proteomic analysis of hippocampus in the rat / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 26-29, 2006.
Article
em Zh
| WPRIM
| ID: wpr-254615
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To initially analyze the protein expression in the rat hippocampus with the proteomics approach.</p><p><b>METHODS</b>Proteins from hippocampal tissue homogenates of the rat were separated by two-dimensional gel electrophoresis(2-DE), and the proteins were stained with colloidal coomassie blue to produce a high-resolution map of the rat hippocampus proteome. Selected proteins from this map were digested with trypsin, and the resulting tryptic peptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The mass spectrometric data were used to identify the proteins through searches of the NCBI protein sequence database.</p><p><b>RESULTS</b>37 prominent proteins with various functional characteristics were identified. The identification of brain protein classes, like metabolism enzymes, cytoskeleton proteins, heat shock proteins, antioxidant proteins, signalling proteins, proteasome-related proteins, neuron-specific proteins and glial-associated proteins. Furthermore, 3 hypothetical proteins which are unknown proteins, so far only proposed from their nucleic acid structure were identified.</p><p><b>CONCLUSION</b>This study provides the first unbiased characterization of proteins of the rat hippocampus and will be used for future studies of differential protein expression in rat models of neurological disorders.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Eletroforese em Gel Bidimensional
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Ratos Sprague-Dawley
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Proteoma
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Proteômica
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Hipocampo
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Metabolismo
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Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article