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Arsenic trioxide induced p15INK4B gene expression in myelodysplastic syndrome cell line MUTZ-1 / 中华血液学杂志
Chinese Journal of Hematology ; (12): 638-641, 2002.
Article em Zh | WPRIM | ID: wpr-261372
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanisms of arsenic trioxide (As(2)O(3)) induced demethylation.</p><p><b>METHOD</b>Methylation of p15INK4B gene in MUTZ-1 cell was detected by PCR using a methylation specific primer (MSP), the expression of P15, DNA methyltransferase (DNMT) 1, DNMT3A and DNMT3B gene by RT-PCR, the As(2)O(3) induced growth inhibition of MUTZ-1 cell by MTT method.</p><p><b>RESULTS</b>P15 gene failed to express in MUTZ-1 cells after methylation. The expression was recovered after the cells exposed to As(2)O(3). As(2)O(3) could significantly down-regulate DNMT3A and DNMT3B but not DNMT1 gene on mRNA level in a dose dependent manner.</p><p><b>CONCLUSION</b>As(2)O(3) could activate the expression of p15 gene by demethylation or/and by inhibiting DNMT3A and DNMT3B gene.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Óxidos / Patologia / Farmacologia / Arsenicais / Síndromes Mielodisplásicas / RNA Mensageiro / Linhagem Celular / Sobrevivência Celular / Regulação da Expressão Gênica / Metilação de DNA Limite: Humans Idioma: Zh Ano de publicação: 2002 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Óxidos / Patologia / Farmacologia / Arsenicais / Síndromes Mielodisplásicas / RNA Mensageiro / Linhagem Celular / Sobrevivência Celular / Regulação da Expressão Gênica / Metilação de DNA Limite: Humans Idioma: Zh Ano de publicação: 2002 Tipo de documento: Article