Human oral carcinoma-associated fibroblasts: its cultivation, purification and identification / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 122-125, 2004.
Article
em Zh
| WPRIM
| ID: wpr-263441
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To separate, cultivate, purify and identify oral carcinoma-associated fibroblasts (CAFs) preliminarily.</p><p><b>METHODS</b>The primary oral CAFs and normal fibrolasts (NFs) of oral mucosa were obtained by tissue culture. Then cells were dissociated by 0.25% trypsin and purified by curettage method and trypsinization. Morphological characteristics were observed under light microscope and electron microscope. The certain proteins were examined by immunohistochemistry (SP method).</p><p><b>RESULTS</b>The third passage purified oral CAFs were maintained. The characteristics of shape and growth of the oral CAFs changed significantly comparing to the NFs. Myofilament and electron dense patch were showed in the oral CAFs by electron microscope. The oral CAFs showed negative staining for cytokeratin, and positive staining for vimentin, alpha-smooth muscle actin and matrix metalloproteinases-2.</p><p><b>CONCLUSIONS</b>There are obvious differences of the morphological characteristics and expression of certain proteins between the CAFs and NFs. The microecology of the oral tumor-host interface might be one of the most important factors affecting the CAFs.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
/
Neoplasias Bucais
/
Separação Celular
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Fibroblastos
/
Métodos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article