Spontaneous differentiation potency of induced pluripotent stem cells into male germ cells in vitro / 中华男科学杂志
National Journal of Andrology
; (12): 8-12, 2011.
Article
em Zh
| WPRIM
| ID: wpr-266221
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression profile of male germ cell-associated genes during the spontaneous differentiation of induced pluripotent stem cells (iPS) and assess the potency of their spontaneous differentiation into male germ cells in vitro.</p><p><b>METHODS</b>Embryoid body (EB) formation was used to promote the spontaneous differentiation of iPS into male germ cells, and the expressions of germ cell-associated genes were detected by real-time PCR and PCR.</p><p><b>RESULTS</b>Real-time PCR and PCR revealed different expression levels of relevant genes at different times of iPS spontaneous differentiation into male germ cells. Each of the 9 genes analyzed exhibited one of the four temporal expression patterns: wavelike increase of Oct4, progressive decrease of Dppa3 and Stra8, wavelike decrease of Dazl, and decrease following initial increase of Tex14, Msy2, Scp1, Scp3 and Akap3.</p><p><b>CONCLUSION</b>Induced pluripotent stem cells express male germ cell-associated genes and male haploid genes during their spontaneous differentiation through EB formation, and have the potency of differentiating into male gametes.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Diferenciação Celular
/
Linhagem Celular
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Perfilação da Expressão Gênica
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Biologia Celular
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Células-Tronco Pluripotentes Induzidas
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Células Germinativas
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Camundongos Endogâmicos ICR
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article