The effect of DNA methyltransferase 1 low expression on the global genome DNA methylation status of 16HBE cell / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 194-197, 2011.
Article
em Zh
| WPRIM
| ID: wpr-272668
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct DNA methyltransferase 1 (DNMT1) low expression 16HBE cell line and observe the variation of cell cycle and global genomic DNA methylation.</p><p><b>METHODS</b>The method of Lenti-virus induced RNA interference was applied to introduce four different shRNA fragment into 16HBE cells. Flow cytometry and 5-mC immunofluorescence methods were used to observe the cell cycle and global DNA methylation status of DNMT1 low expression 16HBE cells.</p><p><b>RESULTS</b>The DNMT1 protein relative expression level of 16HBE-shDNMT1-4 cell line was down regulated about 44% (P < 0.05) compared with the control. No obvious differences of cell cycle and global genome DNA methylation status were observed between the 16HBE and 16HBE-shDNMT1.</p><p><b>CONCLUSION</b>The DNMT1 gene low expression cell is successfully constructed, and there are no obvious changes happened on the cell cycle and global genomic DNA methylation.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Regulação para Baixo
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Ciclo Celular
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Linhagem Celular
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Metilação de DNA
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RNA Interferente Pequeno
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Interferência de RNA
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DNA (Citosina-5-)-Metiltransferases
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Células Epiteliais
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DNA (Citosina-5-)-Metiltransferase 1
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Genética
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2011
Tipo de documento:
Article