HPLC determination of six components in zedoary turmeric oil and its related injections / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 593-597, 2010.
Article
em Zh
| WPRIM
| ID: wpr-281760
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an HPLC method to simultaneously determine six ingredients in zedoary turmeric oil and its related injections.</p><p><b>METHOD</b>HPLC analysis was performed on Waters Symmetry C18 (4.6 mm x 250 mm, 5 microm). The mobile phase was composed of methanol and water solution in gradient elution mode with at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 215 nm.</p><p><b>RESULT</b>The six ingredients were separated well. The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and beta-elemene were 1.74-347.00, 2.38-475.00, 2.49-497.00, 2.07-826.00, 5.05-1009.00, 4.78-955.00 mg x L(-1) (r > or = 0.9996), respectively. The average recoveries were above 95% (RSD <3.0%, n=9).</p><p><b>CONCLUSION</b>The method is accurate, reliable and reproducible, it can be used for the quality control of zedoary turmeric oil and its related injections.</p>
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Base de dados:
WPRIM
Assunto principal:
Óleos Voláteis
/
Química
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Cromatografia Líquida de Alta Pressão
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Curcuma
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Injeções
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Métodos
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article