Influence of ultrasonic processing on the aggregation of PrP-Sc in the brain extracts of the scrapie-infected hamsters / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 118-121, 2004.
Article
em Zh
| WPRIM
| ID: wpr-281839
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To evaluate the influence ultrasonic processing on the aggregation of PrP(Sc) in the brain extracts prepared from the scrapie-infected hamsters, and to seek for the way to prepare lower molecular PrP(Sc) polymer.</p><p><b>METHODS</b>The extracts of infected brains were prepared with a lysis solution, and treated with ultrasonics at various conditions during the different phases. The distribution and aggregation state of PrP(Sc) were analyzed by proteinase K treated. Western blot, and afterwards, quantitatively calculated with a commercially supplied software Image Totaltech.</p><p><b>RESULTS</b>The amount of PrP(Sc) in the supernatant of brain homogenates was 1.29-to 1.58-fold increased with appropriate sonication (15 s for 30 times). At the same conditions of ultrasound, the PrP amount in the supernatant prepared from the scrapie-infected hamster brain was significantly increased, whereas that prepared from healthy animal used as normal control showed little change. Comparative analyses of PrP(Sc) pellets prepared by high-speed centrifugation revealed that about 90% PrP(Sc) released into supernatant after ultrasound processing.</p><p><b>CONCLUSION</b>Appropriate sonication of homogenate of scrapie-infected brain increases the extracted amount of PrP(Sc), being favorable to laboratory diagnosis. Larger molecular PrP(Sc) aggregates can be crashed by ultrasonic processing, engendering lower molecular PrP(Sc) polymers.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Scrapie
/
Sonicação
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Processamento de Imagem Assistida por Computador
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Química Encefálica
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Western Blotting
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Proteínas PrPSc
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Metabolismo
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article