Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray / 中华外科杂志
Zhonghua Wai Ke Za Zhi
; (12): 180-182, 2003.
Article
em Zh
| WPRIM
| ID: wpr-300055
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.</p><p><b>METHODS</b>The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.</p><p><b>CONCLUSIONS</b>cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Transfecção
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Aldeído Redutase
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Resistencia a Medicamentos Antineoplásicos
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Tratamento Farmacológico
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Genética
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Neoplasias Hepáticas
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article