Your browser doesn't support javascript.
loading
Differential gene expression of liver carcinoma cell after transfection of by ARL-1 with microarray / 中华外科杂志
Zhonghua Wai Ke Za Zhi ; (12): 180-182, 2003.
Article em Zh | WPRIM | ID: wpr-300055
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the difference of the gene expression profile and to identify the different expression after transfection of the ARL-1 gene.</p><p><b>METHODS</b>The cDNA probes were synthesized from total RNA of study group and control group, which was differentially hybridized to cDNA chips and confirmed by a gene specific semiquantitative reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Six kinds of gene expression were increased and 9 kinds of gene expression were decreased. The findings were correlated with protein metabolism, signal pathway, metastasis, and drug resistance.</p><p><b>CONCLUSIONS</b>cDNA chips showed that gene expression profile of liver carcinoma cell was changed after transfection of the ARL-1 gene. It is a useful method in understanding the mechanism of drug resistance.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Transfecção / Aldeído Redutase / Resistencia a Medicamentos Antineoplásicos / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Tratamento Farmacológico / Genética / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: Zh Ano de publicação: 2003 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Transfecção / Aldeído Redutase / Resistencia a Medicamentos Antineoplásicos / Análise de Sequência com Séries de Oligonucleotídeos / Perfilação da Expressão Gênica / Tratamento Farmacológico / Genética / Neoplasias Hepáticas Tipo de estudo: Prognostic_studies Limite: Humans Idioma: Zh Ano de publicação: 2003 Tipo de documento: Article