Role of HO/CO in IL-beta induced pancreatic islets apoptosis and the effect of fructose-1, 6-disphosphate / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 548-552, 2009.
Article
em Zh
| WPRIM
| ID: wpr-301564
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the protective role of HO/CO systems in IL-1beta induced islest apoptosis and to explore the mechanisms of the protective effect of fructose-1, 6-disphosphate (FDP).</p><p><b>METHODS</b>The pancreases of the rats were removed to collect islets cells. The cells were incubated with IL-1beta with/or FDP. Cell activity, insulin secretion, HO-1 activity, CO content and apoptotic percentage were detected.</p><p><b>RESULTS</b>HO-1 activity and CO content of the normal control group were low. IL-1beta induced a significant decrease of cell activity and insulin release, flow cytometry analysis showed that apoptotic percentage of islet cells remarkably increased following the addition of IL-1beta, FDP obviously improved the islets cellular activity damaged by IL-1beta, and basic amount of insulin secretion and stimulated by high glucose were improved (P < 0.01). Content of CO and activity of HO-1 were higher in the IL-1beta group than the normal control group (P < 0.05), and there were significant differences between the FDP groups and IL-1beta group. FDP decreased cell apoptotic percentage. Activities of HO-1 and content of CO were higher than that in the IL-1beta group (P < 0.01).</p><p><b>CONCLUSION</b>FDP can attenuate the IL-1beta induced apoptosis of cultured beta cells, the mechanism of which may be improved HO-1 activity and CO content.</p>
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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Monóxido de Carbono
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Células Cultivadas
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Ilhotas Pancreáticas
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Ratos Wistar
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Apoptose
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Secreções Corporais
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Biologia Celular
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Interleucina-1beta
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article