Oriented immobilization of human IgG by protein A on imaging ellipsometry biosensor / 生物工程学报
Chinese Journal of Biotechnology
; (12): 111-114, 2004.
Article
em Zh
| WPRIM
| ID: wpr-305218
Biblioteca responsável:
WPRO
ABSTRACT
The biosensor based on optical imaging ellipsometry, can be used to detect directly, without labeling, the surface concentration of biomolecules on solid surface. The feasibility of using protein A to immobilize antibody on the silicon surface of the imaging ellipsometry biosensor was investigated in this study. The results showed that the anti-IgG immobilized by the protein A on silicon surface could bind effectively human IgG, and the human IgG immobilized on silicon surface by protein A bound more polyclonal antibody molecules than that immobilized on silicon surface directly, suggesting that protein A might block the surface to prevent the absorption of human IgG on surface directly, which might compromise its native configuration. The silicon surface modified with protein A is expected to be used to immobilize a variety of antibodies, as protein A can bind selectively the Fc regions of many mammalian IgG. The combination of imaging ellipsometry and the protein A surface modification has the potential to be developed into immunoassays of high sensitivity.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteína Estafilocócica A
/
Imunoglobulina G
/
Imunoensaio
/
Técnicas Biossensoriais
/
Química
/
Métodos
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article