Expression and switching of TH1/TH2 type cytokines gene in human gliomas / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
; (4): 268-272, 2005.
Article
em En
| WPRIM
| ID: wpr-305403
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas.</p><p><b>METHODS</b>Interleukin (IL)-2 and interferon-gamma represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Thl/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA).</p><p><b>RESULTS</b>The total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues.</p><p><b>CONCLUSION</b>There is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Thl/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.</p>
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Base de dados:
WPRIM
Assunto principal:
Patologia
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Astrocitoma
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Neoplasias Encefálicas
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Regulação Neoplásica da Expressão Gênica
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Citocinas
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Interleucina-4
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Interleucina-6
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Interferon gama
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Interleucina-2
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Interleucina-10
Limite:
Adolescent
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Adult
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Aged
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Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article