Effects of SNP and L-NAME on spermatogenesis in rats / 中华男科学杂志
Zhonghua nankexue
; Zhonghua nankexue;(12): 327-333, 2004.
Article
em Zh
| WPRIM
| ID: wpr-308361
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of nitric oxide(NO) on the DNA ploidy of germ cells and to evaluate the role of NO in modulating spermatogenesis by using SNP, a donor of NO and N-nitro-l-arginine-mythel-ester(L-NAME), an inhibitor of nitric oxide synthese(NOS) in rats physically in vivo.</p><p><b>METHODS</b>Forty adult male, Sprague-Dawley rats (60-70 days) were divided into four groups, and injected ultraperitoneally with one of the following agents (once a day, for 12 days): SNP, L-NAME and SNP + L-NAME with normal saline. Two hours after the last injection the rats were sacrificed. The sera were collected and stored at -70 degrees C for subsequent hormone assay. The concentration of serum testosterone was measured by radioimmunoassay. Serum NOx- (nitrite/nitrate) concentration was measured by Greiss method. DNA of spermatogenic cells was detected by flow cytometry(FCM), and the percentage of 1c, 2c and 4c germ cells calculated.</p><p><b>RESULTS</b>In the SNP treatment group, the serum concentration of NOx- was higher, testosterone concentration was lower and the number of 1c cells was smaller compared with the control group. However, in rats treated with L-NAME, the concentration of NOx- was significantly lower, testosterone concentration was higher and the number of 1c cells was larger compared with the control group(P < 0.01). No changes were observed in the SNP + L-NAME group.</p><p><b>CONCLUSION</b>Enhancing ectogenous NO will suppress spermatogenesis while inhibiting NO productive pathway will promote it.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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Espermatogênese
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DNA
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Nitroprussiato
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Ratos Sprague-Dawley
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NG-Nitroarginina Metil Éster
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Citometria de Fluxo
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Óxido Nítrico
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article