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Chronic pain impairs spatial learning and memory ability and down-regulates Bcl-2 and BDNF mRNA expression in hippocampus of neonatal rats / 中华儿科杂志
Chinese Journal of Pediatrics ; (12): 444-448, 2005.
Article em Zh | WPRIM | ID: wpr-312163
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect and possible mechanism of complete Freund's adjuvant induced chronic pain on later function of learning and memory in neonatal rats.</p><p><b>METHODS</b>Sixty Sprague-Dewley rat pups (10 litters of 6 pups) were randomly divided into control group and chronic pain group (n = 30 in each group). In the chronic pain group, left hind paws of the rats were treated with subcutaneous injection of 20 microl of CFA on postnatal day-2. The control rat pups received normal saline. The hippocampus of rats were separated on postnatal days 10 and 21 (one rat in each group from every litter, n = 10). The expression of Bcl-2 and BDNF mRNA were investigated by RT-PCR. Morris water maze tests were performed on day 21 (one rat in each group from every litter, n = 10).</p><p><b>RESULTS</b>In hidden-platform training of Morris water maze, the mean escape latency of rats in the chronic pain group were longer than that of the control rats. In spatial probe tests, the average percentages of the swimming time and distances in the platform quadrant in the pain group rats were less than those in the control group. There was no significant difference in visible-platform training between the two groups. The Bcl-2 and BDNF mRNA expressions in hippocampus of the pain group rats were lower than those in the control at day 10, but no significant difference at day 21.</p><p><b>CONCLUSION</b>Chronic pain stress induced by CFA impairs the spatial learning and memory function in neonatal rats. These effects might exert through down-regulating Bcl-2 and BDNF mRNA expression in the hippocampus.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Dor / Psicologia / Comportamento Espacial / Estresse Fisiológico / RNA Mensageiro / Regulação para Baixo / Adjuvante de Freund / Doença Crônica / Ratos Sprague-Dawley / Aprendizagem em Labirinto Tipo de estudo: Prognostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Dor / Psicologia / Comportamento Espacial / Estresse Fisiológico / RNA Mensageiro / Regulação para Baixo / Adjuvante de Freund / Doença Crônica / Ratos Sprague-Dawley / Aprendizagem em Labirinto Tipo de estudo: Prognostic_studies Limite: Animals Idioma: Zh Ano de publicação: 2005 Tipo de documento: Article