Formation and identification of virus-like particles of poliovirus type I / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 373-375, 2013.
Article
em Zh
| WPRIM
| ID: wpr-318016
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method to produce virus-like particles (VLP) of poliovirus type I in Saccharomy cescerevisiae to develop potential novel recombinant vaccine against poliovirus type 1.</p><p><b>METHODS</b>The genes of P1 and 3CD of poliovirus type I were optimized, synthesized and inserted into expression vector, which was further transfected into Saccharomy cescerevisiae. The extracts of yeast cells were purified by CsCl density gradient centrifugation after induction and cell lysis.</p><p><b>RESULTS</b>Electrophoresis and sequencing analyses showed that the genes P1 and 3CD of poliovirus type I were successfully inserted into expression vector and encode a protein whose amino acid sequences were identical with wide-type genes of poliovirus type I. Electronic microscopy analysis showed that the VLPs of poliovirus type I could be efficiently formed in Saccharomy cescerevisiae.</p><p><b>CONCLUSION</b>The VLPs of poliovirus type I could be efficiently produced by co-expression of P1 and 3CD genes in Saccharomy cescerevisiae.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Poliomielite
/
Saccharomyces cerevisiae
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Proteínas Virais
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Vírion
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Virologia
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Expressão Gênica
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Vacinas contra Poliovirus
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Poliovirus
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Genética
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Metabolismo
Limite:
Female
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Humans
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Male
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article