Screening of proteins binding to FXR1P using yeast two-hybrid technique / 南方医科大学学报
Journal of Southern Medical University
; (12): 2394-2400, 2009.
Article
em Zh
| WPRIM
| ID: wpr-325108
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen the proteins interacting with FXR1P for functional investigation of FXR1P.</p><p><b>METHODS</b>The yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.</p><p><b>RESULTS</b>The recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.</p><p><b>CONCLUSIONS</b>These results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ligação Proteica
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Autoantígenos
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Biblioteca Gênica
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Proteínas de Ligação a RNA
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Técnicas do Sistema de Duplo-Híbrido
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Estradiol Desidrogenases
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Domínios e Motivos de Interação entre Proteínas
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Ferritinas
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Genética
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Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
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Screening_studies
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article