Optimization of synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2640-2645, 2014.
Article
em Zh
| WPRIM
| ID: wpr-330278
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To optimize the synthetic pathway and fermentation process of yeast cell factories for production of oleanoic acid.</p><p><b>METHOD</b>Using the DNA assembler method, one copy of Glycyrrhiza glabra beta-amyrin synthase (GgbAS), Medicago truncatula oleanolic acid synthase (MtOAS) and Arabidopsis thaliana cytochrome P450 reductase 1 (AtCPR1) genes were introduced into Saccharomyces cerevisiae strain BY-OA, resulting in strain BY-20A. YPD medium with different glucose concentration were then used to cultivate strain BY-2OA.</p><p><b>RESULT</b>Increasing gene copies of GgbAS, MtOAS and AtCPR1 resulted in increased beta-amyrin and oleanolic acid production. The strain BY-2OA produced 136.5 mg x L(-1) beta-amyrin and 92.5 mg x L(-1) oleanolic acid, which were 54% and 30% higher than the parent strain BY-OA. Finally, the titer of oleanolic acid increased to 165.7 mg x L(-1) when cultivated in YPD medium with 40 mg x L(-1) glucose.</p><p><b>CONCLUSION</b>Production of oleanoic acid increased significantly in the yeast strain BY-2OA, which can provide the basis for creating an alternative way for production of oleanoic acid in place of extraction from plant sources.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ácido Oleanólico
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Farmacologia
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Saccharomyces cerevisiae
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Biotecnologia
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Biomassa
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Biologia Celular
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Relação Dose-Resposta a Droga
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Fermentação
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Glucose
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Metabolismo
Idioma:
Zh
Ano de publicação:
2014
Tipo de documento:
Article