Study on effect of weinaokang and bilobalide on autophagy and neurogenesis induced by focal cerebral ischemia reperfusion / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2182-2186, 2013.
Article
em Zh
| WPRIM
| ID: wpr-346418
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the protective effect of the Weinaokang (WNK) and its active compound bilobalide on focal cerebral ischemia reperfusion, and their mechanisms.</p><p><b>METHOD</b>The 60-minute middle cerebral artery occlusion (MCAO) was adopted to establish the 24 h-14 d reperfusion model. The expression of Beclin-1 was detected by the Western blotting technique. The transmission electron microscopy was used to observe ultrastructural changes. Neurogenesis was detected by the immunofluorescence staining.</p><p><b>RESULT</b>WNK (20, 10 mg x kg(-1), ig) or its active compound bilobalide (10, 5 mg x kg(-1), ig) could promote the generation of mature neurons (BrdU(+) -MAP-2+) at the ischemic side, and inhibit expression of autophagy-related gene Beclin-1, so as to reduce the neuron injury induced by focal cerebral ischemia reperfusion.</p><p><b>CONCLUSION</b>WNK and its active compound bilobalide can inhibit neuron autophagy and improve neurogenesis in ischemic peripheral area, suggesting that neurogenesis may be the intervention target for WNK to promote self-repairing of ischemic area.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Autofagia
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Medicamentos de Ervas Chinesas
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Traumatismo por Reperfusão
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Isquemia Encefálica
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Ratos Sprague-Dawley
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Ciclopentanos
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Ginkgolídeos
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Tratamento Farmacológico
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2013
Tipo de documento:
Article