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Detection of luxS gene of quorum sensing signal system in periodontal pathogen / 中华口腔医学杂志
Article em Zh | WPRIM | ID: wpr-346779
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the presence and distribution of luxS gene in quorum sensing signal system in the periodontal pathogens.</p><p><b>METHODS</b>The total DNA of Porphyromonas gingivalis (Pg), Fusobacterium nucleatum (Fn), Actinobacillus acitinomycetimcomtans (Aa) were extracted. The presence of luxS was detected by polymerase chain reaction (PCR). The products of PCR were detected by electrophoresis, sequenced and identified by a Blast search of the GenBank database.</p><p><b>RESULTS</b>Electrophoresis, sequencing and Blast searching indicated that the PCR products of Pg were highly consistent with the luxS gene in GenBank. The sequencing result of Fn was also identified with the target gene. The PCR product of Aa was the same as reference through electrophoresis.</p><p><b>CONCLUSIONS</b>Pg, Fn, Aa contain luxS gene. Further studies may be required to investigate the functions of luxS in the periodontal pathogens.</p>
Assuntos
Texto completo: 1 Base de dados: WPRIM Assunto principal: Liases de Carbono-Enxofre / Proteínas de Bactérias / Transdução de Sinais / Regulação Bacteriana da Expressão Gênica / Fusobacterium nucleatum / Aggregatibacter actinomycetemcomitans / Porphyromonas gingivalis / Percepção de Quorum / Genética / Metabolismo Tipo de estudo: Diagnostic_studies Idioma: Zh Ano de publicação: 2009 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Liases de Carbono-Enxofre / Proteínas de Bactérias / Transdução de Sinais / Regulação Bacteriana da Expressão Gênica / Fusobacterium nucleatum / Aggregatibacter actinomycetemcomitans / Porphyromonas gingivalis / Percepção de Quorum / Genética / Metabolismo Tipo de estudo: Diagnostic_studies Idioma: Zh Ano de publicação: 2009 Tipo de documento: Article