Role of CD44 on airway inflammatory response in rats with asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 142-145, 2009.
Article
em Zh
| WPRIM
| ID: wpr-347981
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of cell adhesion molecule CD44 in the lung on airway inflammatory response in rats with asthma.</p><p><b>METHODS</b>Thirty-two Sprague-Dawley rats were randomly divided into normal control and asthma groups. Asthma was induced by repeated inhalation of ovalbulium. CD44 expression in the lung was detected by semi-quantitatively reverse transcription-polymerase chain reaction (RT-PCR) and immuno-histochemical staining 1 week and 2 weeks after ovalbulium challenge. Differential leukocytes (mononuclear phagocytes, neutrophils, eosinophils, and lymphocytes) in bronchoalveolar lavage fluid (BALF) were counted.</p><p><b>RESULTS</b>CD44 expression in the lung increased 1 week after ovalbulium challenge (<0.05) and increased more significantly 2 weeks after ovalbulium challenge (<0.01) compared with that in the control group. The percentages of lymphocytes and eosinophils in BALF increased, while the percentage of BALF mononuclear phagocytes decreased significantly 1 week and 2 weeks after ovalbulium challenge in the asthma group compared with those in the control group (<0.05). An increased percentage of neutrophils was found 1 week after ovalbulium challenge in the asthma group compared with the control group (<0.05). CD44 expression in the lung was positively correlated with the percentages of lymphocytes and eosinophils in BALF in the asthma group 1 week and 2 weeks after ovalbulium challenge, in contrast, lung CD44 expression was negatively correlated with the percentage of mononuclear phagocytes in the asthma group 1 week after ovalbulium challenge.</p><p><b>CONCLUSIONS</b>CD44 was over-expressed in the lung and closely related to inflammatory infiltration in rats with asthma. CD44 may play an important role in the development of airway inflammatory in asthma.</p>
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Base de dados:
WPRIM
Assunto principal:
Fisiologia
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Asma
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RNA Mensageiro
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Líquido da Lavagem Broncoalveolar
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Ratos Sprague-Dawley
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Receptores de Hialuronatos
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Biologia Celular
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Alergia e Imunologia
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Eosinófilos
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Genética
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article