Determination of acetylharpagide in Ajuga decumbens by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 289-290, 2006.
Article
em Zh
| WPRIM
| ID: wpr-350958
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of the quantitative determination of acetylharpagide in Ajuga decumbens.</p><p><b>METHOD</b>The chromatographic conditions were as follows: a Phenomenex Luna C18 column was used, the mobile phase was composed of acetontrile-water (15:85), the flow rate was 1.0 mL x min(-1) and the UV absorbance detection was set at 197 nm.</p><p><b>RESULT</b>Linearity of acetylharpagide was in the range of 0.6-3.6 microg (r = 0.9993), and the average recovery and RSD were 99.13% and 2.48%, respectively.</p><p><b>CONCLUSION</b>The contents of acetylharpagide ranged 0.40%-6.39% in A. decumbens. The method was simple, accurate and sensitive.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plantas Medicinais
/
Piranos
/
Medicamentos de Ervas Chinesas
/
Química
/
Reprodutibilidade dos Testes
/
Cromatografia Líquida de Alta Pressão
/
Sensibilidade e Especificidade
/
Ajuga
/
Métodos
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Ano de publicação:
2006
Tipo de documento:
Article