Protective effects of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea and its mechanism / 药学学报
Acta Pharmaceutica Sinica
; (12): 690-694, 2005.
Article
em Zh
| WPRIM
| ID: wpr-353427
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the protective effect of ligustrazine against photoreceptor cell injury induced by N-methyl-N-nitrosourea (MNU) in Sprague-Dawley (SD) rats.</p><p><b>METHODS</b>Ligustrazine injections of different doses were injected intraperitoneally into 47-day female SD rats once a day and a single intraperitoneal injection of MNU 60 mg x kg(-1) was given to 50-day rats. At different intervals after MNU treatment,the animals were sacrificed. The apoptotic index of photoreceptor cells was calculated by TUNEL labeling at 24 h following MNU treatment; peripheral retinal damage was evaluated based on retinal thickness at the d 7 after MNU treatment, and the expression of c-jun and c-fos genes was detected by RT-PCR technique.</p><p><b>RESULTS</b>Ligustrazine injection could remarkably increase total thickness of peripheral retina and decrease apoptotic index of photoreceptor cells induced by MNU in a dose-dependent manner. Compared with MNU-treated rats, the gene expression of c-jun and c-fos was time-dependently down-regulated in ligustrazine-treated group.</p><p><b>CONCLUSION</b>Ligustrazine injection partially protects against MNU-induced retinal damage by down-modulating the expression of c-jun and c-fos genes to inhibit apoptosis of photoreceptor cells.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Farmacologia
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Células Fotorreceptoras
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Plantas Medicinais
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Pirazinas
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Retina
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Química
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Genes jun
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Genes fos
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Ratos Sprague-Dawley
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article