Effect of ketamine on transient outward potassium current of isolated human atrial myocytes / 药学学报
Acta Pharmaceutica Sinica
; (12): 849-852, 2010.
Article
em Zh
| WPRIM
| ID: wpr-354565
Biblioteca responsável:
WPRO
ABSTRACT
The effects of ketamine on transient outward potassium current (I(to)) of isolated human atrial myocytes were investigated to understand the mechanism of part of its effects by whole-cell patch-clamp. Atrial myocytes were enzymatically isolated from specimens of human atrial appendage obtained from patients under going cardiac valve displacing. Ito is recorded in voltage-clamp modes using the patch-clamp technique at room temperature. Currents signals were recorded by an Axopatch 200B amplifier with the Digidata 1322A-pClamp 9.0 data acquisition system. Ketamine decreased I(to) of human atrial myocytes in a dose-dependent manner. The current-voltage curve was significantly lowered, 30, 100, 300, and 1000 micromol x L(-1) ketamine decreased respectively I(to) current density about (13.62 +/- 0.04)%, (38.92 +/- 0.05)%, (72.24 +/- 0.10)% and (83.84 +/- 0.05)% at the potential of 50 mV, with an IC50 of 121 micromol x L(-1). The I(to) activation curve, inactivation curve and the recovery curve were not altered by ketamine. So, ketamine concentration-dependently decreased I(to) of human atrial myocytes.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Fisiologia
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Canais de Potássio
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Técnicas de Patch-Clamp
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Biologia Celular
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Miócitos Cardíacos
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Relação Dose-Resposta a Droga
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Átrios do Coração
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Ketamina
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Anestésicos Dissociativos
Limite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
Idioma:
Zh
Ano de publicação:
2010
Tipo de documento:
Article