Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles
Journal of Veterinary Science
; : 37-41, 2006.
Article
em En
| WPRIM
| ID: wpr-36291
Biblioteca responsável:
WPRO
ABSTRACT
A total of 22 Salmonella enterica serotype Enteritidis (S. Enteritidis) strains isolated from human and chicken were subjected to DNA fingerprinting by repetitive sequence PCR using ERIC and BOX primers, antibiotic resistance and plasmid patterns. Both ERIC and BOX PCR amplification data revealed a highly genetic homogeneity between isolates from human and chicken except one isolate, which originated from chicken and showed a different DNA band pattern from others. Eleven of 22 S. Enteritidis isolates (50%) were resistant to more than one antibiotics and characterized by 5 resistance patterns. The most common pattern was penicillin resistant (63.6%). Only one isolate from chicken showed a multiple drug resistance patterns to 4 antibiotics. All 22 S. Enteritidis isolates harbored more than two plasmids with eight different plasmid profiles including two to six plasmids with approximate molecular size ranging from 1.9 to 21 kb. A band of 15 kb size was detected in all isolates tested, however, the band sizes smaller than 15 kb were found only in isolates from chicken.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
/
Doenças das Aves Domésticas
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Salmonella enteritidis
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Intoxicação Alimentar por Salmonella
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DNA Bacteriano
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Testes de Sensibilidade Microbiana
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Galinhas
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China
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Reação em Cadeia da Polimerase
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Surtos de Doenças
Limite:
Animals
/
Humans
País como assunto:
Asia
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article