Development of an Improved Animal Model of Overactive Bladder: Transperineal Ligation versus Transperitoneal Ligation in Male Rats
The World Journal of Men's Health
; : 137-144, 2016.
Article
em En
| WPRIM
| ID: wpr-39525
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
We compared a transperineal ligation model and a transperitoneal ligation model in male rats to determine which animal model of overactive bladder (OAB) was more useful based on cystometrography, estimations of oxidative stress, and measurements of pro-inflammatory cytokine levels. MATERIALS ANDMETHODS:
Male rats were randomly divided into three groups (n=15 in each) the control group, the transperineal ligation group, and the transperitoneal ligation group. Four weeks after the ligation procedure, cystometrography was performed and oxidative stress, pro-inflammatory cytokine levels, and histologic changes were evaluated. Oxidative stress was assessed by measuring 8-hydroxy-20-deoxyguanosine and superoxide dismutase, and pro-inflammatory cytokine activity was investigated by measuring levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α.RESULTS:
The transperineal model led to results similar to those observed for the transperitoneal model, namely (1) increased voiding frequency and reductions in the non-voiding contraction interval and the maximal vesical pressure, (2) increased levels of oxidative stress markers, (3) increased pro-inflammatory cytokine levels, and (4) fibrotic changes in the bladder tissue.CONCLUSIONS:
We suggest that the transperineal procedure can be used as an alternative OAB model in male rats.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Superóxido Dismutase
/
Bexiga Urinária
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Obstrução do Colo da Bexiga Urinária
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Interleucina-8
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Interleucinas
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Estresse Oxidativo
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Modelos Animais
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Bexiga Urinária Hiperativa
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Ligadura
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Necrose
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
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Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article