Molecular cloning and sequencing of the cDNA of the soluble human leukocyte antigen-G1 / 免疫学杂志
Immunological Journal
; (12): 81-84, 2001.
Article
em Zh
| WPRIM
| ID: wpr-433893
Biblioteca responsável:
WPRO
ABSTRACT
Objective To construct a recombinant plasmid pcDNA3-sHLA-G1 expressing soluble HLA-G1. Methods Total cell RNA was extracted from the cell line Jeg-3 and the cDNA was amplified by RT-PCR; The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3-sHLA-G1 had been constructed successfully. Conclusion In this study, the recombinant plasmid pcDNA3-sHLA-G1 had been constructed successfully.
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Base de dados:
WPRIM
Idioma:
Zh
Ano de publicação:
2001
Tipo de documento:
Article