miR-200c regulates migration of breast cancer cell BT549 by targeting Slug / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 304-307, 2015.
Article
em Zh
| WPRIM
| ID: wpr-460410
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To investigate the effect on the expression of Slug for the trasfection of miR-200c combined with the research on the ability of migration of breast cancer cell BT549.Methods:Chemically synthesized miR-200c mimic was trasfected into BT549 cells,which have high metastatic potential.The effect on the ability of migration of breast cancer cell BT549 for the transfection of miR-200c was analyzed by Transwell migration assay and Wound healing assay.The expression of Slug and E-cadherin mRNA was detected by Real-time PCR.The expression of Slug protein was detected by Western blot.Results:Transfection with miR-200c mimic significantly down-regulated the expression of Slug as compared with the control group (P<0.05).BT549 cell tranfected with miR-200c mimic had lower levels of migration capacity than cells in the control group (P<0.05).Conclusion:miR-200c inhibits Epithelial-mes-enchymal transition by suppressing Slug leading to down-regulation of migration capacity of breast cancer cell BT549.
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Base de dados:
WPRIM
Idioma:
Zh
Ano de publicação:
2015
Tipo de documento:
Article