NOD2 up-regulates the autophagy and inhibites the proliferation and migration of SCC-1 5 cells / 实用口腔医学杂志

ABSTRACT

Objective:To investigate the affects of NOD2 on rapamycin (Rap)induced autophagy and on the proliferation and mi-gration of tongue squamous carcinoma SCC-1 5 cells.Methods:① Synthesized NOD2 over-expression plasmid and NOD2-shRNA were transfected into SCC-1 5 cells respectively.②Normal control SCC-1 5 cells,NOD2 over-expression cells and NOD2-shRNA cells were treated with Rap to induce autophagy.Then,the expression of LC3 and Beclin-1 was examined by Western blot.Cell pro-liferation was tested by MTT assay.Cell migration was assessed by wound healing assay.Results:①After Rap treatment,the expres-sion of protein LC3-Ⅱand Beclin-1 in NOD2 over-expression cells increased(P <0.05)and in NOD2-shRNA cells were suppressed (P <0.05).② Compared with control group,the proliferation and migration ability were decreased in NOD2 over-expression cells (P <0.05),but in NOD2-shRNA cells the proliferation and migration ability were increased(P <0.05).Conclusion:NOD2 can up-regulate the autophagy and suppress the proliferation and migration of tongue squamous SCC-1 5 cells.