Clinical significance of expression of human cytomegalovirus pp67-mRNA in transplant recipients / 中华器官移植杂志
Chinese Journal of Organ Transplantation
; (12)1996.
Article
em Zh
| WPRIM
| ID: wpr-539249
Biblioteca responsável:
WPRO
ABSTRACT
ObjectiveTo establish a new method for diagnosis of active human cytomegalovirus (HCMV) i nfection and disease in transplant recipients.MethodsThe expression of HCMV pp67-mRNA in blood was detected by the nucleic acid sequence-based amplification (NASBA), in comparison to detection of pp65 HCMV a ntigen in white blood cells.ResultsIn 50 patients subject to transplantation, 26 were positive for pp65 (≥1/50 000 WBC). Among them, 16 were positive for pp67. In 9 cases of CMV with symptoms, b oth pp65 and pp67 were positive. In the patients negative for pp65, pp67 was not positive. The sensitivity, specificity, positive predictive value and negative predictive value were 100?%, 88?%, 56?% and 100?%, respectively.ConclusionDetection of pp67-mRNA by NASBA is a rapid objective and accurate approach for the diagnosis of HCMV infection.
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Base de dados:
WPRIM
Idioma:
Zh
Ano de publicação:
1996
Tipo de documento:
Article