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Empirical study of treatment of peritoneal carcinomatosis with a combination of adenoviral p53 gene therapy and thymosin ?1 / 肿瘤研究与临床
Article em Zh | WPRIM | ID: wpr-544034
Biblioteca responsável: WPRO
ABSTRACT
Objective To evaluate the cooperative effect of intraperitoneal adenoviral p53 gene therapy together with thymosin ?1 against peritoneal carcinomatosis. Methods KM mouse model bearing peritoneal carcinomatosis was established using H22 cell line. 48 hours after inoculation, adenoviral-mediated p53 gene was administrated intraperitoneally alone, or combined with thymosin ?1 within 7 days. The abdominal girth, body weight, tumor cell number, apoptosis and cell cycle were tested using methods of cell counting and flow cytometry. Results Adenoviral p53 gene transfer alone arrested H22 cell in G0/G1. In combination therapy group, ascitic fluid was obviously halted, tumor cells in peritoneal cavity decreased in number and ascites formation, dead cell ratio was elevated. Conclusion Adenoviral-mediated p53 gene could block cell proliferation. Intraperitoneal adenoviral p53 gene therapy combined with thymosin ?1 showed synergism for the lethal damage and inhibition for peritoneal carcinomatosis.
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Texto completo: 1 Base de dados: WPRIM Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article