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Effect on radiation-induced apoptosis of HeLa cells by inhibiting expression of NF-?B / 中国癌症杂志
China Oncology ; (12)2006.
Article em Zh | WPRIM | ID: wpr-545156
Biblioteca responsável: WPRO
ABSTRACT
Background and purpose: NF-?B is activated by tumor necrosis factor,some chemotherapeutic agents,and ionizing radiation.The activation of NF-?B could result in inhibition of apoptosis.NF-?B,regulated by PDTC(pyrrolidine dithiocarbamate),is a specific inhibitor of NF-?B.The purpose of our study was to explore the impact of inhibiting expression of NF-?B on radiation-induced apoptosis of uterine cervix cancer HeLa cells.Methods:Inhibition of NF-?B in HeLa cells was performed by treatment with 100 ?mol/L PDTC for 2 hours.Cells were irradiated at 0,2,4 and 6 Gy with or without PDTC.The expression of NF-?B in nuclear was determined by western blot,and apoptosis was evaluated by using the TUNEL assay.Cell proliferation was evaluated by using MTT assay.Results:NF-?B activation was induced by radiation and inhibited by PDTC.Inhibition of radiation-induced NF-?B activation resulted in inhibiting cell proliferation(P
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Texto completo: 1 Base de dados: WPRIM Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Idioma: Zh Ano de publicação: 2006 Tipo de documento: Article