Inhibition of PI3K/PKB signal pathway improves chemotherapeutic effect on gastric carcinoma cell lines / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-564725
Biblioteca responsável:
WPRO
ABSTRACT
Aim To observe whether the inhibition of PI3K/PKB signal pathway by LY294002[PI3K pathway inhibitor] could improve the sensitivities of human gastric carcinoma cell line SGC7901 and SGC7901/VCR to anti-cancer drugs.Methods The sensitivities of SGC7901 and SGC7901/VCR to chemotherapeutic drug VCR were detected by MTT.The MDR1 and XIAP mRNA expression levels were evaluated by semi-quantitative RT-PCR,and their protein levels were detected by immunocytochemistry.The PKB and phospho-PKB protein levels were detected by Western blot and the apoptosis ratio was detected by flow cytometry.Results 2?10-5mol?L-1LY294002 enhanced the sensitivities of SGC7901 and SGC7901/VCR cells to VCR(P
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WPRIM
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article