Effects of extract from Narcissus tazettavar. chinensis on proliferation and apoptosis of human multiple myeloma cell line ARH-77 / 中草药
Chinese Traditional and Herbal Drugs
; (24)1994.
Article
em Zh
| WPRIM
| ID: wpr-574392
Biblioteca responsável:
WPRO
ABSTRACT
Objective To study the effects of extract from Narcissus tazetta var. chinensis on proliferation and apoptosis of human multiple myeloma cell line ARH-77. Methods The survival rate of cells were tested by MTT assay when cells were affected by 1.25, 2.5, 5, 10, 20 ?g/mL of the extract for 3 d. After being induced by 10 ?g/mL of the extract for 3 d, apoptotic cells were observed with fluorescence stain; changes of he csll cycle were analyzed by Flow Cytometry; the number and shape of nucleolar organizer region (NOR) were tested by AgNOR assay and submicroscopic structure were observed by transmission electron microscope. Results The extract from N. tazetta var. chinensis could significantly inhibit the proliferation and reduce the survival rate of ARH-77 cells (P
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WPRIM
Idioma:
Zh
Ano de publicação:
1994
Tipo de documento:
Article