The apoptosis and expression of apoptosis related proteins in the developing mouse stomach / 重庆医科大学学报

ABSTRACT

Objective: To observe the apoptosis and expression of apoptosis related proteins Bcl-2,Bax and P53 during the developing mouse stomach.Methods: Serial sections of fetal mice aged 11～15 days were made.The density of apoptotic bodies(DAB) in the epithelium was measured with stereological method.The expression of apoptosis related proteins Bcl-2,Bax and P53 in the epithelium were detected with the SABC method of immunocytochemistry,and image analysier was used to detect the products-intensity of each section.Results: ① The peak-expression of DAB in the epithelium was on the 14 th and 15 th day.② The peak-expression of Bcl-2 in the epithelium was on the 11 th and 13 th day,the peak-expression of Bax in the epithelium was on the 14 th day,the peak-expression of P53 were on the 11 th and 14 th day.Conclusion: ① Bcl-2 may have a role in suppressing cell apoptosis in the epithelium of stomach,Bax may have a role in promoting cell apoptosis.P53 do not have close relationship with the control of epithelial cells apoptosis in fetus mouse stomach.