Determination of free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC / 中成药

ABSTRACT

AIM: To detect free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC. METHODS: A method for determining gallic acid in leaves of Acer Truncatum Bunge.The operating conditions HPLC were Diamonsil C_18 column(5 ?m,4.6 mm?250 mm) at room temperature,methanol-0.025 mol/L phosphoric acid solution(15∶85) as mobile phase at a flow of 1.0 mL/min and UV detection at 272 nm. RESULTS: The contents of free and hydrolysable gallic acid were 0.045% and 1.546%,repectively.The relative standard deviations of free and hydrolysable gallic acid were 1.06% and 2.43%,respectively. CONCLUSION: The contents of free and hydrolysable gallic acid in Acer Truncatum Bunge in different periods are different.