Effects of E-cadherin gene transfection on bladder cancer cell growth and invasion / 第三军医大学学报

ABSTRACT

Objective To observe the effects of E cadherin (ECD) on bladder cancer cell growth and invasion using gene transfection technique. Methods Human E cadherin gene was transfected into a highly invasive and metastatic bladder cancer cell line Ts3L. Cell growth curves, clone formation rate, in vitro tumor cell infiltrating ability, and cell cycle distribution were determined, respectively. Results Speed of bladder cancer cell growth decreased significantly to 35 7% after ECD gene transfection. Apoptotic cell rate in ECD transfected cells was markedly higher than that of the control cells. The percentage of superploid cells in Ts3L/ECD cells was significantly reduced than that in the control cells. Clone formation rate of ECD transfected cells, detected by soft agarose gel clone formation test, was significantly decreased as compared with that of the control cells ( P