THE APPLICATION OF PRE-EMBEDDING DOUBLE-LABELED IMMUNO-ELECTRON MICROSCOPIC METHOD IN THE NEUROANATOMICAL STUDIES / 解剖学报

ABSTRACT

Objective The co existence of two kinds of neurotransmitters in the cell bodies or axon terminals and the relationship between a neurotransmitter and its relevant receptor were observed at the ultrastructural level. Methods The pre embedding double labeled immuno electron microscopic techniques were used by peroxidase immunohistochemistry combined with immunogold silver labeled method. Results In the sections of the striatum,a large number of the SP like immunoreactive(\|LI) axon terminals(immunoperoxidase reaction products)and SP receptor(SPR)\|LI neuronal cell bodies and dendrites(immunoglold silver grains)were seen in the present study.Some SP\|LI axon terminals were observed to form symmetric axon somatic or axon dendritic synaptic contacts with SPR\|LI neuronal cell bodies and dendritic profiles.On the other hand,in the sections of caudal spinal trigeminal nucleus,a substantial number of axon terminals of the two kinds of the vesicular glutamate transporters,the differentiation associated Na + dependent inorganic phosphate cotransporter(DNPI)and vesicular glutamate transporter of type1(VGluT1),were labeled by peroxidase immunohistochemistry and immunogold silver grains.Some axon terminals were labeled by both DNPI and VGLuT1 immunoreaction formed asymmetric synaptic contact with non labeled dendrite profile.Conclusion\ The pre embedding double labeled immuno electron microscopic technique has the advantage of higher sensitivity and greater preservation of the antigen in the many tissues.Thus,the present study favors the application of this technique to study the co existence of two kinds of the neurotransmitters in the cell bodies or axon terminals and analyse the relationship between a neurotransmitter and its relevant receptor in the field of the neuroanatomy.