Biosynthesis analysis of hederagenin pathway and its construction in yeast cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1844-1850, 2018.
Article
em Zh
| WPRIM
| ID: wpr-690704
Biblioteca responsável:
WPRO
ABSTRACT
Hederagenin is an effective constituent of many medical plants, such as Clematidis Radix, and has a wide range of applications in anti-tumor, anti-inflammatory, antidepressant, hepatoprotective antibacterial, et al. In order to obtain the efficient production of yeast cells for hederagenin,we successfully cloned and screened out a P450 gene MdMA02 from Malus×domestica which can catalyze oleanolic acid C-23 oxidation with our developed plug and play platform. Its amino acid homology is only 32% as compared to characterized CYP72A68v2. By transforming MdMA02 to the oleanolic acid-producing strain BY-OA, a hederagenin-producing strain was constructed and hederagenin's titer could achieve 101 mg·L⁻¹ using high cell density fermentation, which was 337 times higher than in shake flasks culturing. This study provides a basis for further research on promoting the creation of oleanane-type pentacyclic triterpenoids biosynthetic pathway analysis and relative cell factories construction.
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Base de dados:
WPRIM
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article