Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes
The Korean Journal of Physiology and Pharmacology
; : 201-206, 2004.
Article
em En
| WPRIM
| ID: wpr-727923
Biblioteca responsável:
WPRO
ABSTRACT
Mitochondrial ATP-sensitive potassium (mitoKATP) channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of mitoKATP channels remains unclear. In this study, we investigated the subunit composition of mitoKATP channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while mitoKATP channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of mitoKATP channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands (~4, ~6, and ~0 kDa) were detected, and a specific ~6-kDa immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of mitoKATP channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Potássio
/
Western Blotting
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Imunofluorescência
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Microscopia Confocal
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Precondicionamento Isquêmico
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Células Musculares
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Miócitos Cardíacos
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Canais KATP
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Fluorescência
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Mitocôndrias
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article