PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions
Mycobiology
; : 7-10, 2001.
Article
em En
| WPRIM
| ID: wpr-729312
Biblioteca responsável:
WPRO
ABSTRACT
Based on the rDNA ITS sequences data, specific primer set for PCR detection of wood-decaying fungus Phellinus linteus was designed. The length of PCR products using designed primer set(SHF and SHR) was about 540 bp. Among 11 species, 17 isolates of Phellinus spp. including Phellinus linteus, P. pomaceus, P. spiculosus, P. baumi, P. pini, P. igniarius, P. gilvus, P. biscuspidatus, P. weirii, P. johnsonianus, P. robutus, and P. igniarius, seven isolates of Phellinus linteus showed about 540 bp-sized single band. This molecular technique could offer a useful tool for detecting and identifying Phellinus linteus.
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Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
DNA Ribossômico
/
Reação em Cadeia da Polimerase
/
Fungos
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article