Effect of Erzhiwan on Invasion and Metastasis of Human Colon Cancer Cells / 中国实验方剂学杂志

ABSTRACT

Objective: To investigate the effect of Erzhiwan (EZW) on the invasion and metastasis of human colon cancer HCT116 cells. Method: The abilities of invasion (number of transmembrane cells) and migration (relative width of 48 h scratch) were observed by Transwell assay. Western blot was used to detect vascular endothelial growth factor (VEGF) and E-cadherin protein, respectively. Result: ① Transwell results showed that compared with the blank control group, the number of model piercing cells in each drug group was decreased (PPPPPPPP-1 L-OHP combined with 10% EZW group was lower than that in L-OHP group (PP-1 L-OHP combined with 10% EZW group and L-OHP group increased the expression of E-cadherin protein significantly. Compared with L-OHP group, the expression of E-cadherin protein was the highest (PConclusion: EZW can inhibit the invasion and metastasis of colon cancer HCT116 cells, and the best effect is achieved after combined with L-OHP. This may be related to the decrease of VEGF protein expression and the increase of E-cadherin protein expression after combination with L-OHP.