Difference analysis of expression and role of Galectin-3 in ocular venous malformation after urea treatment / 中华整形外科杂志

ABSTRACT

Objective@#To evaluate the role of Galectin-3 protein and mRNA in venous malformation of ocular region after urea injection treatment.@*Methods@#108 cases with venous malformation of ocular region were randomly divided into two groups: 53 cases did not receive urea injection before operation, the other 55 cases were treated by injection of urea. The expressions of Galectin-3 protein and mRNA were detected using by immunohistochemical staining and in situ hybridization in 108 cases of venous malformation of ocular region and 20 normal tissue from distal surgical margin.The difference of Galectin-3 expression with and without the intervention of urea was analyzed, as well as the relationship between Galectin-3 and clinicopathological features.@*Results@#The positive rate of Galectin-3 protein and mRNA were 52.83%(28/53) and 58.49%(31/53) in venous malformation of ocular region tissue without the urea intervention respectively, whereas the epithelial cells of ocular region tissue from the distant cutting margin had weak reactivity which were 10%(2/20) and 15%(3/20) respectively (P<0.05). With the urea intervention, the positive value were 23.64%(13/55) and 30.91%(17/55) (P>0.05). Compared with no urea intervention treatment group, the positive rate expression of Galectin-3 protein and mRNA decreased markedly in urea intervention group (P<0.05).@*Conclusions@#Galectin-3 may promote neovascularization and play an important role in the development of the ocular venous malformation. Meanwhile, it may also serve as a valuable detect marker in venous malformation evolution.Urea intervention can control and relieve the Galectin-3 protein and mRNA for the development of the ocular venous malformations.