Effects of serine/threonine-protein kinase B-Raf-activated long-chain non-coding RNA on apoptosis and autophagy in thyroid carcinoma cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 747-753, 2018.
Article
em Zh
| WPRIM
| ID: wpr-813201
Biblioteca responsável:
WPRO
ABSTRACT
To investigate the effect of serine/threonine-protein kinase B-Raf (BRAF)-activated long-chain non-coding RNA (lncRNA-BANCR) on apoptosis and autophagy in thyroid carcinoma cells and the underlying mechanisms.
Methods:
RT-PCR was used to detect the expression of lncRNA-BANCR in thyroid carcinoma and normal thyroid tissues. The association between lncRNA-BANCR and clinicopathological data was analyzed in patients with thyroid cancer. Cell counting kit-8 (CCK-8) was used to detect the effect of lncRNA-BANCR on the proliferation of thyroid cancer cells. The effect of lncRNA-BANCR on the apoptosis of thyroid carcinoma cells was detected by flow cytometry. Transwell invasion assay was used to detect the effect of lncRNA-BANCR on the invasive ability of thyroid cancer cells. Western blot was used to detect the changes of autophagy proteins LC3-I and LC3-II after the lncRNA-BANCR expression was suppressed.Results:
Compared with normal thyroid tissues, the expression level of lncRNA-BANCR in thyroid carcinoma tissues was elevated (P<0.05). The expression of lncRNA-BANCR was positively related to the pathological stage of thyroid carcinoma and the lymph node metastasis. Inhibition of lncRNA-BANCR expression attenuated the proliferation and invasion ability of thyroid cancer cells (both P<0.05); but the apoptosis was enhanced (P<0.05); the expression levels of autophagy protein LC3-I and LC3-II were also increased (P<0.05).Conclusion:
The expression level of lncRNA-BANCR affects the proliferation, invasion and apoptosis of thyroid cancer cells through modulation of autophagy behavior.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Patologia
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Serina
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Autofagia
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Treonina
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Glândula Tireoide
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Neoplasias da Glândula Tireoide
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Regulação Neoplásica da Expressão Gênica
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Apoptose
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Biologia Celular
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Proteínas Proto-Oncogênicas B-raf
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2018
Tipo de documento:
Article