JAK/STAT3SignalingPathwayIsInvolvedinPromotingtheProliferationand MigrationofBronchialSmoothMuscleCellsInducedbyInterleukin-17 / 中山大学学报(医学科学版)
Journal of Sun Yat-sen University(Medical Sciences)
; (6): 379-386, 2019.
Article
em Zh
| WPRIM
| ID: wpr-817790
Biblioteca responsável:
WPRO
ABSTRACT
@#:【Objective】Toinvestigatetheeffectsofinterleukin-17(IL-17)ontheproliferationandmigrationof bronchialsmoothmusclecells(BSMC)andtheroleofJAK/STAT3signalingpathwayinthisprocess.【Methods】BSMC weretreatedwithdifferentconcentrationsofIL-17fordifferenttimestodeterminethebestoftheexperimentalcondition. ThenMTTassaywasusedtodetectcellviability.CellproliferationstatesweredetectedbyBrdUstaining,andthecell cyclewasassessedbyPIstainingusingaflowcytometer.Transwellcellmigrationassaywasfurtherusedtodetectcell migrationability.TheexpressionofJAK,p-JAK,STAT3andp-STAT3inBSMCafterbeingtreatedwithIL-17was detectedbyWesternblotting.JAK/STAT3signalingpathwayspecificblockerAG490wasusedtoinvestigatetheroleof JAK/STAT3signalingpathwayinIL-17-inducedBSMCproliferationandmigration.TheeffectsofIL-17oncellproliferation, migration and JAK/STAT3 signaling pathway related protein expression were evaluated after blocking the JAK/STAT3 signaling.【Results】IL-17enhancedtheproliferation(P<0.05),promotedthecellcycletransitions(P<0.05)andsig⁃nificantlyincreasesthemigrationability(P<0.05)inBSMC.ThisprocesswasaccompaniedbytheenhancementofJAK/ STAT3signalingpathwayinBSMC(P<0.05).InhibitionofJAK/STAT3signalingpathwayalleviatedBSMCproliferation andmigrationinducedbyIL-17(P<0.05) .【Conclusions】JAK/STAT3signalingpathwayparticipatesinthestimulation processofIL-17ontheproliferationandmigrationofBSMC.AG490inhibitstheenhancementofJAK/STAT3signaling pathwayinBSMCinducedbyinterleukin-17.
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WPRIM
Idioma:
Zh
Ano de publicação:
2019
Tipo de documento:
Article