Screening, identification and classification of an antimitosis marine bacterium from Eastern China Sea / 第二军医大学学报

ABSTRACT

Objective: To screen, identify and classify an antimitosis marine bacterium strain 32-11-2-2 of Eastern China Sea. Methods: The marine bacteria were isolated with the infinite dilution and plate streak technique and screened for antimitotic active strains by Pyricularia Oryzae model. The active strain 32-11-2-2 was subjected to morphological, physiological and biochemical study, and was classified by the analysis of the 16S rDNA sequence. Results: A total of 421 strains of marine bacteria were isolated from marine water and sediment samples; 54 of them had antimitotic activity. The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bacillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis. Conclusion: The strain 32-11-2-2 has been identified to be a Bacillus subtilis adapted to the oceanic environment.