HPLC fingerprint of Xiangsha Liujun Pills from different manufacturers / 中草药

ABSTRACT

Objective: To establish an HPLC fingerprint of Xiangsha Liujun Pills (XLP), and to apply this method to determining 49 batches of samples from 16 different manufacturers for quality evaluation, supporting the foundation of quality control system of XLP. Methods: The HPLC separation was performed on a Sepax Bio-C18 reversed-phase column in gradient mode of acetonitrile-0.1% acetic acid with UV detection at 260 nm. The column temperature was kept at 30℃, and the flow rate of mobile phase was 1.0 mL/min. The fingerprint similarities and cluster analysis based on the chromatographic peaks in detected samples were obtained. Results: The HPLC fingerprint of XLP was developed firstly. Seventeen common fingerprint peaks were indicated and attributed to raw materials, and 16 of them were identified by comparison with reference standards and UPLC-Q-TOF-MS. The analyzed samples were geographically classified into four classes with the similarities between 0.617-0.988. Conclusion: Significant differences of quality in XLP on the market are observed. The method is simple, reliable, and suitable for the quality control of XLP, supplying helpful information for the comprehensive evaluation of XLP.