Simultaneous Determination of Amino Acids, Nucleosides and Nucleobases in Different Parts of Metaplexis japonica(Thunb.) Makino Processed by Different Methods by UFLC-QTRAP-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an UFLC-QTRAP-MS/MS method for simultaneous determination of 15 kinds of amino acids and 12 kinds of nucleosides and nucleobases in different parts of Metaplexis japonica(Thunb.) Makino processed by different methods. METHODS: The analysis was carried out on a Waters XBridge Amide column(2.1 mm×100 mm, 3.5 μm) with elution by mobile phase of 0.2% formic acid in acetonitrile-0.2% formic acid in water at a flow rate of 0.6 mL·min-1. The column temperature was maintained at 30 ℃. The target compounds were analyzed by positive ion multiple reaction monitoring(MRM) mode. RESULTS: Twenty-seven multiple constituents showed good linearity(r>0.997 3) in the ranges of the tested concentrations. The average recoveries of the 27 components were 98.17%-100.75% with relative standard deviations of 0.90%-2.49%. CONCLUSION: The established method is accurate and precise, which provides a reliable solution for selecting the parts and the optimal processing method of Metaplexis japonica(Thunb.)Makino.