Anti-inflammatory effects of Nelumbo leaf extracts and identification of their metabolites
Nutrition Research and Practice
; : 265-274, 2017.
Article
em En
| WPRIM
| ID: wpr-89160
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND/OBJECTIVES:
Nelumbo leaves have been used in traditional medicine to treat bleeding, gastritis, hemorrhoids, and halitosis. However, their mechanisms have not been elucidated. MATERIALS/METHODS:
The present study prepared two Nelumbo leaf extracts (NLEs) using water or 50% ethanol. Inflammatory response was induced with LPS treatment, and expression of pro-inflammatory mediators (inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 and nitric oxide (NO) and prostaglandin E₂ (PGE₂) productions were assessed. To determine the anti-inflammatory mechanism of NLEs, we measured nuclear factor-κB (NF-κB) activity. Major metabolites of NLEs were also analyzed and quantified.RESULTS:
NLEs effectively reduced the expression and productions of pro-inflammatory mediators such as IL-1β, IL-6, TNF-α, PGE₂, and NO. NLEs also reduced NF-κB activity by inhibiting inhibitor of NF-κB phosphorylation. Both extracts contained catechin and quercetin, bioactive compounds of NLEs.CONCLUSIONS:
In this study, we showed that NLEs could be used to inhibit NF-κB-mediated inflammatory responses. In addition, our data support the idea that NLEs can ameliorate disease conditions involving chronic inflammation.Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fosforilação
/
Quercetina
/
Água
/
Dinoprostona
/
Catequina
/
Interleucinas
/
Interleucina-6
/
Óxido Nítrico Sintase
/
Nelumbo
/
Etanol
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article