Direct protection of cultured neurons from ischemia-like injury by minocycline / 대한해부학회지
Anatomy & Cell Biology
; : 325-331, 2010.
Article
em En
| WPRIM
| ID: wpr-93237
Biblioteca responsável:
WPRO
ABSTRACT
Minocycline, a tetracycline antibiotic, is now known to protect cells via an anti-inflammatory mechanism. We further explored this effect using an in vitro model of ischemia-like injury to neurons. Coculturing neurons with microglia, the brain's resident immune cell, modestly increased cell death due to oxygen and glucose deprivation (OGD), compared to neurons alone. Treatment of cocultures with minocycline decreased cell death to a level significantly lower than that of neurons alone. Treatment of cocultures with minocycline or inhibitors of various immune mediators, also led to decreased cell death. Importantly, treatment of neuron cultures without added microglia with these same inhibitors of tissue plasminogen activator, matrix metalloproteinases, TNF-alpha and inducible nitric oxide synthase as well as minocycline also led to decreased cell death. Thus, anti-inflammatory treatments appear to be directly protective of neurons from in vitro ischemia.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Oxigênio
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Tetraciclina
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Fator de Necrose Tumoral alfa
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Morte Celular
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Ativador de Plasminogênio Tecidual
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Microglia
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Técnicas de Cocultura
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Metaloproteinases da Matriz
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Óxido Nítrico Sintase Tipo II
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Glucose
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article