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1.
J Membr Biol ; 248(3): 431-42, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25837994

RESUMO

Staphylococcus epidermidis has emerged as one of the major nosocomial pathogens associated with infections of implanted medical devices. The most important factor in the pathogenesis of these infections is the formation of bacterial biofilms. Bacteria grown in biofilms are more resistant to antibiotics and to the immune defence system than planktonic bacteria. In these infections, the antimicrobial therapy usually fails and the removal of the biofilm-coated implanted device is the only effective solution. In this study, three proteomic approaches were performed to investigate membrane proteins associated to biofilm formation: (i) sample fractionation by gel electrophoresis, followed by isotopic labelling and LC-MS/MS analysis, (ii) in-solution sample preparation, followed by isotopic labelling and LC-MS/MS analysis and (iii) in-solution sample preparation and label-free LC-MS/MS analysis. We found that the commensal strain S. epidermidis CECT 231 grown in biofilms expressed higher levels of five membrane and membrane-associated proteins involved in pathogenesis: accumulation-associated protein, staphylococcal secretory antigen, signal transduction protein TRAP, ribonuclease Y and phenol soluble modulin beta 1 when compared with bacteria grown under planktonic conditions. These results indicate that a commensal strain can acquire a pathogenic phenotype depending on the mode of growth.


Assuntos
Proteínas da Membrana Bacteriana Externa/metabolismo , Biofilmes , Staphylococcus epidermidis/fisiologia , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/metabolismo , Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Espectrometria de Massas em Tandem , Regulação para Cima , Virulência , Fatores de Virulência/genética , Fatores de Virulência/metabolismo
2.
Mater Sci Eng C Mater Biol Appl ; 50: 1-11, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25746238

RESUMO

Microbial growth on medical and technical devices is a big health issue, particularly when microorganisms aggregate to form biofilms. Moreover, the occurrence of antibiotic-resistant bacteria in the clinical environment is dramatically growing, making treatment of bacterial infections very challenging. In search of an alternative, we studied a novel antimicrobial surface coating based on micro galvanic elements formed by silver and ruthenium with surface catalytic properties. The antimicrobial coating efficiently inhibited the growth of the nosocomial pathogens Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Enterococcus faecium as demonstrated by the growth inhibition on agar surface and in biofilms of antibiotic resistant clinical E. faecalis, E. faecium, and S. aureus isolates. It also strongly reduced the growth of Legionella in a drinking water pipeline and of Escherichia coli in urine. We postulate a mode of action of the antimicrobial material, which is independent of the release of silver ions. Thus, the novel antimicrobial coating could represent an alternative to combat microbial growth avoiding the toxic side effects of high levels of silver ions on eukaryotic cells.


Assuntos
Antibacterianos/farmacologia , Resistência Microbiana a Medicamentos , Viabilidade Microbiana/efeitos dos fármacos , Microbiologia da Água , Doenças Transmitidas pela Água/microbiologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Técnicas de Cultura Celular por Lotes , Catálise/efeitos dos fármacos , Contagem de Colônia Microbiana , Desinfecção , Resistência Microbiana a Medicamentos/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Prata/farmacologia , Espectrometria por Raios X , Abastecimento de Água
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