Multiple Spectroscopic, Docking and Cytotoxic Study of a Synthesized 2,2' Bipyridin Phenyl Isopentylglycin Pt(II) Nitrate Complex: Human Serum Albumin and Breast Cancer Cell Line of MDA-MB231 as Targets.

In the present study, the biological activities of a new synthesized Pt(II)-complex, 2,2' bipyridinphenyl isopentylglycin Pt(II) nitrate was investigated via its interaction with the most important blood carrier protein of human serum albumin (HSA), using fluorescence and Far-UV circular dichroism (CD) spectroscopic techniques and also molecular docking. Moreover, cytotoxicity activity of the complex was studied against breast cancer cell line of MDA MB231 using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The Pt(II)-complex has a strong ability to quench the intrinsic fluorescence of HSA through a static quenching mechanism. According fluorescence quenching data, the binding parameters of the interaction were calculated and showed that hydrophobic interaction has an important role. The molecular docking results in coherent with fluorescence measurements illustrated that Pt(II) complex can bind to HSA at one position that located in the hydrophobic cavity of groove between drug site I and II. Also, experimental data on driving force in binding site was confirmed whereas theoretical results demonstrated Pt(II) complexinteract to HSA by hydrophobic interaction. Far-UV-CD results showed that Pt(II)-complex induced an increasing in the content of α-helical structure of the protein and stabilized it. Also, MTT assay represented growth inhibitory effect of the complex toward the breast cancer cell line.

Frequency of amount and axis of astigmatism in subjects of Rawalpindi, Pakistan.

OBJECTIVE: To assess the frequency of amount and axis of astigmatism with respect to age and gender. METHODS: The prospective, observational study was carried out from September 2009 to January 2010 at Armed Forces Institute of Ophthalmology, Rawalpindi. Patients from 5-70 years of age were included from both genders with no previous history of eye abnormality, surgery or eye pathology. An auto refractor-keratometre was used for measurement. Descriptive statistics were used to analyse data on SPSS 15. RESULTS: Among the total 288 patients, with-the-rule astigmatism (n = 21; 55%) had greater frequency in the 26-35 years sub-group. In terms of gender, 88 (48%); against-the-rule in 81 (44%); and oblique astigmatism in 14 (7.6%). In females, with-the-rule astigmatism was found in 52 (50%); against-the-rule in 36 (34.2%); and oblique in 17 (16%). The frequency of astigmatism > or = 0.25 ranged from 0.8% (n = 1) to 74% (n = 28) across all age groups. The amount of astigmatism which was noted to be the most common and prominent in the sample was < 1D. CONCLUSION: The amount and axis of astigmatism varied within the age sub-groups and within the gender.

Screening Programs for Hearing Assessment in Newborns and Children.

Objective In this study, we aimed to assess the incidence of hearing loss in the pediatric population through otoacoustic emission (OAE) and brainstem evoked response audiometry (BERA) and to analyze the possible etiological factors responsible for it. Material and methods A retrospective observational study was conducted in the Otolaryngology (ENT) and Gynecology and Obstetrics Departments at the Jinnah Postgraduate Medical Centre and National Institute of Child Health in Karachi, Pakistan between July 2019 and October 2019. The convenient sampling technique was used to select the patients. The final sample size consisting of newborns and children was 108. Initially, screening procedures were undertaken for newborns to detect permanent or fluctuating, bilateral or unilateral, and sensory or conductive hearing loss, averaging 30-40 dB or more in the frequency region, which indicated potential issues related to speech recognition (approximately 500-4,000 Hz). The screening of newborns involved the use of non-invasive, objective physiologic measures that included OAEs and/or auditory brainstem response (ABR). The children with hearing impairment then underwent BERA; thereafter, further investigations were performed to confirm the defects found on BERA testing. Results Of the 108 cases, 96 had normal hearing on OAE screening, and 12 were found to have hearing loss on the OAE test. Further testing was carried out on BERA for 12 cases that had been detected to have hearing loss on OAE, and BERA showed normal hearing for five cases whereas seven were found to have hearing loss. Of the seven patients with hearing loss on the BERA test, five were diagnosed with cochlear deafness, and two had retrocochlear deafness. Conclusion Our present study concludes that in order to avoid any hearing problems in infants, OAE hearing screening and diagnostic BERA screening programs should be carried out in all the hospitals of Pakistan to assess newborn hearing at an early age.

A case of non-surgical pneumoperitonium: gas under the diaphragm.

A 58-year-old woman presented with an unexpected episode of vomiting and an acute abdomen. The patient mentioned a vague history of decreased appetite dyspepsia and constipation. Abdominal X-Ray revealed gas under diaphragm. An Open laparotomy was carried out to evacuate the free gas trapped under the diaphragm. The condition is almost always associated with perforation of abdominal viscera and accumulation of air during surgical or gynaecological procedures or peritoneal dialysis. In the reported case, laparotomy revealed no sign of perforation in GIT, uterine fundus or fistulas and nor did the have patient have any history of surgical or gynaecological procedures. This lead to suggestion of spontaneous or non-surgical pneumoperitonium which is extremely rare. Extensive investigations revealed no known cause pneumoperitonium making our case rare and unique.

The Prevalence of Psychiatric Disorders in Children and Adolescents in Hamadan Province, West of Iran.

BACKGROUND: There are numerous reports regarding increasing childhood and adolescent mental health problems. The aim of this study was to determine the prevalence of psychiatric disorders in Hamadan Province, west of Iran from July 2016 to May 2017. STUDY DESIGN: A cross-sectional study. METHODS: The sample included 1025 Hamadan residents selected using multistage cluster sampling. Psychiatric disorders were assessed by semi-structured psychiatric interview Kiddie-Sads-Present and Lifetime Version (K-SADS-PL). The data were analyzed using the SPSS software. We used the multivariable logistic regression to predict the Odds Ratios (ORs). RESULTS: The prevalence of total psychiatric disorder was 8.6%. Psychiatric disorders in boys were higher than girls (12.6% and 4.9%, respectively). The psychiatric disorders were most prevalent in 6-9 yr old age group (11%). The prevalence of behavioral disorder was 3.8% with attention deficit hyperactivity disorder (ADHD) as the most prevalent case (2.0%). The prevalence of anxiety disorder was 2.8% in which the highest prevalence belonged to separation anxiety disorder (SAD) (1.1%). The prevalence of neurodevelopment disorder was 1.5% with the highest prevalence of 1% observed in epilepsy. The prevalence of mood disorder was 1.1% with the depressive disorder as the most prevalent one (1.0%). The prevalence of enuresis was 2.7%. The most common comorbidities were anxiety and mood disorders 5(50.0%). CONCLUSION: The prevalence of these disorders in Hamadan was less than the prevalence in other cities of Iran. These findings can be helpful for large-scale planning for children and adolescents.